Abstract:
This study investigated the effects of ADS (6-aminopurine hemisulphate salt) and GSH
(glutathione) on callus formation and tissue browning in unfertilized ovary cultures of
coconut (Cocos nttcifera Z.). The primary objective was to enhance the efficiency and
success of the ovary culture protocol by addressing issues related to tissue browning and
low callus initiation rates.
For ADS, a concentration of 70 mgfl resulted in the highest number of callus formations
but exhibited similar browning to the control. After thorough analysis, concentrations of
60 mg/l and 65 mg/l were deemed suitable for achieving low browning and high callus
formation. Higher concentrations led to increased browning, suggesting that further
research with lower concentrations may enhance callus formation while reducing
browning.
In the case of GSH, a concentration of 95 mgll was found to be optimal for callus
formation with low browning. Increasing the concentration of GSH further reduced
browning. Therefore, higher concentrations of glutathione warrant further investigation.
However, according to the mean rank, glutathione was not as effective as the control due
to a higher browning index compared to the control.
