Abstract:
Coconut water waste is a valuable resource since it can be used sustainably in several
sectors, such as agriculture, the manufacturing of biofuel, and as a nutrient-rich
substrate for microbial development. The objective of this study was to compare the
growth and survival of Saccharomyces cerevisiae in coconut water waste incorporated
medium and conventional potato dextrose broth (PDB) medium. To conduct the study,
growth media were prepared using a series of coconut water waste, and sterilized. Then,
medias were inoculated with yeast cultures and subsequently incubated under
controlled conditions. Numerous parameters were assessed, such as temperature, pH,
optical density, transmittance, and cell viability. An analysis of the nutritional
composition of the coconut water revealed considerable quantities of minerals and
carbohydrates that are favorable to yeast development. One-way ANOVA and Tukey
tests showed a significant difference between the treatments. The treatment (T5:
Coconut wastewater I00yo), with the greatest quantity of coconut water waste,
demonstrated the highest growth metrics and cell survival across all the other treatments
that were studied. In particular, the T5 exhibited significantly higher optical density and
cell viability compared to other treatments, indicating optimal growth conditions for
Saccharomyces cerevisiae. Whlle PDB w:rs associated with high pH levels, T5
outperformed in terms of cell viability and count, suggesting that coconut water waste
can be an effective and sustainable alternative to conventional growth media for yeast
cultivation, aligning well with the observed trends in this study. To maximize growth
rates and investigate the long-term stability of yeast cultures in media based on coconut
water, more investigation is advised. This study contributes to the field of sustainable
biotechnology by proposing the efficient utllization of agro-industrial waste for
microbial cultivation, potentially reducing both production costs and environmental
impact.
