Abstract:
Banana (Musa acuminata) is a tropical fruit, which has high intemational demand due to
its nutrition value and potential use in various industries. Micropropagation of Banana
focused on mass production of Banana plants in in vitro using artificial nutrient medium
providing fast and efficient way to produce large number of plants with consistence
characteristics. Micropropagation is highly relying on the artificial chemical and nutrient
components for the growth and proliferation of explant. This study investigates the effect
of different carbon sources on the growth and development of Amban banana (Musa
acuminata) shoot tips in tissue culture.
Banana shoot tips were selected from the third sub culturing cycle obtained from the Fruit
Research and Development Institute (FRDD. An artificial nutrient media was prepared
for the establishment of shoot tips using Murashige and Skoog (MS) media included
Glucose and Sucrose, Ferrous sodium EDTA, micronutrients, macronutrients, myinositol,
Vitamin, BAP 5 mg/I, NAA 0.2 mgll and agar. The media was prepared with
nine different treatments, where glucose and sucrose were tested at four different
concentrations such as media with glucose20 gll, glucose 30 gll, glucose 40 gll, glucose
50 g/l and media with sucrose20 fl|, sucrose 30 gll, sucrose 40 gll. sucrose 50 g/l and a
control was included without any carbon source. The data was analyzed under four
criteria such as number of shoots, weight of shoots, and height of shoot. These data were
analyzed by considering two subculture cycles.
The findings from ANOVA and LSD tests confirmed that the significant effects of the
different carbon sources, highlighting glucose (30 g/l) as the most effective treatment for
both shoot number and weight, while sucrose (20 gll) was identified as the most effective
for shoot height. The study concludes with recommendations for the optimal use of
carbon sources in an in-vitro propagation of Amban banana shoot tips, emphasizing the
robustness of glucose (30 g/l) for shoot multiplication and weight, and sucrose (20 gll)
with 5 mgll BAP and2 mg/l NAA containing MS media for shoot elongation.
