Abstract:
Micropropagation is a highly efficient technique for the large-scale production of
genetically unifotm, disease-free plants. This study aimed to develop an optimized in
vitro propagation protocol for the 'Malee Pink' pomegranate variety by evaluating
surface sterilization, shoot multiplication, and root induction conditions. Effective
surface sterilization is crucial for reducing microbial contamination while maintaining
explant viability. The best sterilization treatment was identified as 2o/o Clorox for 2
minutes, significantly minimizing bacterial and fungal contamination without causing
tissue damage. Pre-tests were conducted to assess phenolic exudation, bacterial
contamination, and fungal infection, ensuring optimal conditions for culture initiation.
Shoot multiplication was achieved using Murashige and Skoog (MS) medium
supplemented with 3 mglL of 6-Benzylaminopurine (BAP), which promoted vigorous
shoot proliferation while preventing abnormal growth. For root induction, gibberellins
supplementation was optimized to achieve a high rooting percentage and the
development of a strong root system, essential for successful acclimatization. The
effectiveness of different treatments was assessed based on survival rates,
contamination levels, and shoot vigor. The optimized protocol significantly enhances
the efficiency of in vitro propagation, providing a scalable and reliable method for
commercial pomegranate production. Future research may explore antioxidant
treatments to rnitigate phenolic oxidation and improve plantlet health, further
supporting mass propagation and ensuring the availability of high-yielding, diseasefree
'Malee Pink'plants for commercial cultivation.
